PV92 Alu Insertion Lab Report - 1100 Words

PV92 Alu Insertion (Lab Report Sample) Content: PV92 Alu InsertionName:Institution:IntroductionIt is important to note that alignment sequence was done based on the instructions given in the laboratory manual. The chosen database was denoted as "Genome (all assemblies top - level)à ¢Ã¢â€š ¬Ã‚ . This was the most preferred choice since "Genome (all assemblies top-level, Annotation Release 106)à ¢Ã¢â€š ¬Ã‚ ; was not there amongst given list of choices. Thus, results differ slightly from the answers given.Step 5On which human chromosome did BLAST find a sequence alignment?Answer: Chromosome 16Homo sapiens Chromosome 16, GRCh38.p2 Primary Assembly; Sequence ID: ref|NC_000016.10| Length: 90338345, Number of Matches: 2. Hit score for the reverse primer was 49.1 bits (26), e-value was 2e-04, identities 26/26 which is equivalent to a 100% match with zero gaps introduced. The forward primer hit score was 47.3 bits (25) with an e-value of 9e-04, identities 25/25 which equivalents to a 100% match therefore, and no gaps we re introduced. Figure 1: http://blast.ncbi.nlm.nih.gov/Blast.cgiWhat is the name of the gene with which the PV92 forward primer aligns (see all headed "featuresà ¢Ã¢â€š ¬Ã‚ )?Answer:The name of the gene is Cadherin 13 isoform 2. The human chromosome number 16 has high segments of duplicate nucleotide sequences. Based on Martin, et al., (2004), the chromosome contains a total of 78,884,754 base pairs as reported in the completed Human Genome Project. Manual annotation demonstrates that there are a total number of 880 protein-coding sequences, which have been verified by 1,670 transcripts alignments, 19 RNA gene sequences. It also contains 341 pseudogenes as well as 3 RNA pseudogenes (Martin, et al., 2004).One of the genes is the cadherin gene. It encodes for calcium-dependent as well as membrane associated glyco-proteins. The uniqueness of encoded protein is that it does not have the pro-sequence as well as the tripeptide HAV binding recognition sequences which are characteristic o f many types of cadherins (Martin, et al., 2004).How good is the alignment (how many nucleotides align with the 25-long primer)?Answer:25/25 100%, it is clear that there was no gaps which were introduced during the alignment. Thus the 25-long primer completely matched the query sequence. More information is as highlighted in Figure 2 below. Figure 2: http://blast.ncbi.nlm.nih.gov/Blast.cgiWhat is the e-value, also called "expect," for this alignment?Answer:2e-04 for the reverse primer and 9e-04 for the forward primer: Figure 2 above shows the query results for both. What is the "Range" of the alignment (i.e., the numbered base-pairs, starting from one end of the chromosome) with which the forward primer sequence aligns)?The answers given before the search are in contrary with what was determined later. The previous results suited that of Homo sapiens chromosome 16, alternate assembly CHM1_1.1 sequence, as shown in Figure 3 below (Martin, et al., 2004). A summary of alignment results is as follows;Forward PrimerA hit score of 47.3 bits (25), e-value of 9e-04, identification level at 25/25 (0%), no gaps, and range of between 84323157 to 84323181. Reverse PrimerA hit score of 49.1 bits (26), e-value of 2e-04, identification level of 26/26 (100%), and no gaps. Its range was determined to lie between 84323563 to 843235588. Figure 3: http://blast.ncbi.nlm.nih.gov/Blast.cgiAnswer:However, the sequence to be used for this report was specified as that from the Homo sapiens chromosome 16, GRCh38.p2 Primary Assembly, gi|568815582|ref|NC_000016.10|. The answer to this particular question will be based on this sequence. Figure 4 below shows the derived results. Forward PrimerThe forward primer recorded a hit score of 47.3 bits (25) with the sequence in the database. It had an e-value of 9e-04, 100% identification value with no introduction of gaps when aligning. Its range was found to lie between 82878195 to 82878219. Length is 25.Reverse PrimerThe reverse primer recorde d a hit score of 49.1 bits (26), an e-value of 2e-04 (100%) with no gaps introduced during the alignment process. The range for this sequence was determined to lie between 82878585 to 82878610. Length is 26.Figure 4: http://blast.ncbi.nlm.nih.gov/Blast.cgiAnswer:How many base-pairs long is the PCR fragment that would be expected from use of these two primers?Answer:The entire mapped sequences ranges from 82878195 to 82878610. As per the instructions given on the laboratory, the lowest range for the forward primer was 82878195 and the greatest range for the reverse primer was identified as 82878610. Please refer to Figure 4 as shown above. The number of base pairs which can be derived from both primers will be 82878610 minus 82878195, which is gives a total and DNA product of 415 base pairs in length. Step 8What tissue is this gene associated with?Answer:Cadherins are proteins which are overly expressed in the heart tissues as compared to other tissues such as the ...

Diversity Management Focuses On The Heterogeneity Inside...

Diversity management focuses on the heterogeneity inside the organizations. Diversity management was a response to the common situation when companies started to hire employees of various age, nationality, race, religion etc. It is based on the principle that the differences between people do not have to prevent them from working together. This is directly connected with the concept of ethical behavior. Generally ethics is defined as a moral code which is accepted in a concrete society, though there are cases when ethical does not mean everything that is legal. For example, slavery was legal in the United States society, but from now it is not the best manifestation of the ethical behavior (Schermerhorn, Davidson, Poole, Woods, 2012).†¦show more content†¦Promoters of affirmative action maintain that these measures can assist to equalize the starting conditions and even out the playing field between those that are affiliated with minority groups and those not. This is contra dicted by the fact that, if diversity management aspires to create a situation, wherein every individual feels valued and has the opportunity to improve himself. Then, all actions must be directed to creating equal opportunities for every individual, not only for members of relative individual groups. According to Paul Burstein, there are three competing views on affirmative actions those are: ïÆ' ± therapeutic actions which are measures which are necessary for the elimination of past discrimination; ïÆ' ± delicate balance these consist of providing help to the minority without harming the majority; ïÆ' ± aid without preferences meaning that nobody receive favorable treatment according to their identity with a specific group; In the United States there was a shift from the therapeutic actions to the aid without preferences course of action, the coining of the diversity management concept is a manifestation of this trend. Whilst the US reconsiders its approach to affirmative action, British companies, especially among the public sector, continue to engage in

Models of Addiction Essay - 822 Words

Models of Addiction SUBS 505 Models of Addiction The three models of addiction examined in this week’s readings include the medical model, the psychosocial model, and the disease of the human spirit model. The medical model â€Å"rests on the assumption that disease states are the result of a biological dysfunction, possibly one on the cellular or even molecular level† (Doweiko, 2012, p. 333). Many consider this model and â€Å"maintain that much of human behavior is based on the interaction between the individual’s biological predisposition and the environment† (Doweiko, 2012, p. 333). Individuals under this model view free will â€Å"as an illusion† (Doweiko, 2012, p. 333). There is controversy regarding this model as â€Å"to the degree to which the†¦show more content†¦In considering all the models that propose to offer insight in the factors to consider with addiction, â€Å"each perspective fails to completely explain all of the facets of the SUDs adequately† (Doweiko, 201 2, p. 352). The third model of substance use disorders presented is the disease of the human spirit. This model suggests that as we enter the burdens and trials of life and become ungrounded with pain or voids in our lives we allow ourselves to feel pity and open ourselves up to our inwardly sinful nature. â€Å"It is at this point that some recoil in horror and become spiritual narcissists: self-centered, unwilling to see any reason to deny the â€Å"self† any desire or pleasure† (Doweiko, 2012, p. 357). This model believes that all individuals â€Å"all start out with hope, faith and fortitude† but when exposed to the ills of the world some â€Å"turn to chemicals to fill the perceived void within or to ease their pain† (Doweiko, 2012, p. 361). When considering spiritual bankruptcy and void in the life of an addict, despair comes to mind. When an individual feels despair they are unable to consider anything else but what is happening in the present and often appear desperate. Literature supports that â€Å"people are also spiritual beings who are either actively or passively involved in a relationship with a Higher Being† (Doweiko, 2012, p. 353). When a person is spiritually bankrupt they have lost moral direction and often begin to make poor, self-pleasingShow MoreRelatedAddiction Models988 Words   |  4 PagesSubstance Use Behavior Addiction is regarded as having a multitude of causations and contributing factors. No single specific component or model can accurately predict a person’s substance use behavior (Clinton amp; Scalise, 2013). Substance use disorders are a reflection of the impact of person-specific biological, psychological, and social influences. Understanding an individual’s spiritual beliefs and the role spirituality plays in a person’s susceptibility to addiction is also necessary. If aRead MoreAddiction : The Moral Model Of Addiction Essay2483 Words   |  10 Pagesdoes the disease model of addiction differ from the moral model of addiction? The disease model of addiction and the moral model of addiction provide completely different explanation for the tendency of substance abuse. The disease model of addiction predates to 1784 when the American physician Benjamin Rush published a pamphlet which discussed alcoholism in medical terms and outlined treatments for what he considered was a â€Å"disease† (Atkins, 2014, p. 52). This model of addiction generally arguesRead MoreThe Medical Model Of Addiction1413 Words   |  6 Pages Drug and Alcohol Treatment in America has been based on the Medical Model of Treatment. According to Wikipedia, the medical model of addiction is rooted in the philosophy that addiction is a disease and has biological, neurological, genetic, and environmental sources of origin. Treatment includes potential detox with a 28 day or more stay at a residential treatment facility. 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The model is based on the idea that many factors can play an equal role in contributing to a disease. Some have criticized the model due to the fact that some diseases involve few psychological or social factors. Although this is rarely the case for addiction counseling, some still believeRead MoreA Comparison of Psychoanalytic Formulations of Addiction and Cognitive Models of Addiction666 Words   |  3 PagesIn this paper I will be comparing and contrasting the Psychoanalytic formulations of addiction and the Cognitive models of add iction. According to Dennis L. Thombs, â€Å"people tend to get psychoanalysis and psychotherapy mixed up. Psychotherapy is a more general term describing professional services aimed at helping individuals or groups overcome emotional, behavioral or relationship problem† (119). According to Thombs and Osborn, â€Å"Cognitive refers to the covert mental process that are described byRead More The Etiology of Addiction Disease Model Essay examples1522 Words   |  7 PagesAddiction is like all behaviours â€Å"the business of the brain†. Addictions are compulsive physical and psychological needs from habit-forming sustenances like nicotine, alcohol, and drugs. Being occupied with or involved in such activities, leads a person who uses them again and again to become tolerant and dependent eventually experiencing withdrawal. (Molintas, 2006). Addictive drugs cause dopamine neurons to release dopamine, the pleasure hormone. The narcotics disable the neurons that wouldRead MoreAddiction : A Serious Problem Essay1559 Words   |  7 PagesAddiction is a very serious problem in today’s society. It is the goal of counselors to help those who suffer from addictions. There are many different models that attempt to explain what addiction is, and how someone gets addicted. There many different views about addiction. â€Å"Historically addiction has been understood in various ways- a sin, a disease, a bad habit-each a reflection of a variety of social, cultural and scientific conceptions(Hammer et al., 2012 p. 713). While there are many differentRead MoreTheoretical Concepts. There Are Many Theoretical Concepts1208 Words   |  5 Pages Theoretical concepts There are many theoretical concepts to explain addictions, a few that I see being used in NA and AA meetings is the Disease Model, Social Learning Model, and Cognitive Behavioral Therapy. In both of the meetings I attended the members stood up and admitted they are powerless over their addiction. This falls in with the Disease Model. According to the disease model the addiction is in the individual’s brain. Therefore, the addict is â€Å"incapable of controlling their ownRead MoreFriends of Youth and Lakeside Milam Recovery Centers Essay1342 Words   |  6 Pagesvery detailed tour and explanation of their inpatient program. Treatment Treatment for addictions is multivariate. When looking at appropriate treatments it is important to consider the treatment setting, treatment approach, what is important when treating varying age groups, as well as treating addiction and mental health simultaneously. All of these factors play a large part in treating patients with addiction; appropriate treatment may different between patients. Treatment Setting When touring

Supernatural in Hamlet free essay sample

Supernaturalism is a manifestation of intellectual curiosity. Modernity has prohibited such curiosity with technological inquisition. But while it can be avoided phylogenetically, it cannot be avoided ontogenetically. With modern theatre, this aspect of mythology and the treatment of the supernatural elements, bear a direct inclination towards politics. But this tendency to profess political ideas is not modern but penetrates deep into the ancient world. Shakespeare’s tragedies are flagship plays of all such constitutions. His treatment of supernaturalism, whether in Julius Caesar or Hamlet, has both the political and personal elements. â€Å"Far from being a feudal poet†, observes Wyndham Lewis in The Lion and the Fox,1 â€Å"the Shakespeare that ‘Troilus and Cressida’, ‘The Tempest’, or even ‘Cariolanus’ shows us is much more a bolshevik (using this little word popularly) than a figure of conservative romance. † As a dramatist, Shakespeare was bound to provide entertainment for his audience. But, in Hamlet, his hatred for mere entertainment becomes evident in one of Hamlet’s famous dialogues: HAMLET. Be not too tame neither, but let your own discretion be your tutor. Suit the action to the word, the word to the action, with this special observance: that you o’erstep not the modesty of nature. For anything so overdone is from the pur- pose of playing, whose end, both at the first and now, was and is to hold as ‘twere the mirror up to nature, to show virtue her own feature, scorn her own image, and the very age and body of the time his form and pressure. Now this overdone, or come tardy off, though it make the unskilful laugh, cannot but make the judicious grieve; censure of the which one must in your allowance o’erweigh a whole theatre of others (3. 2. 16-28). This, is what precisely, happens in Hamlet: a play within the play is staged, an extensive decoy, which would reveal the true nature of the king and would be a charge against the rotten state of Denmark. This being established, that a veil works through the subject of the play, where nothing is what it appears to be, we would try to look into the treatment and nature of the ‘supernatural’ elements in the First Act of the play and the role it plays. The supernatural elements in Hamlet have become a risky boomerang from the viewpoint of productions and filmmaking. The risk is much more subjective than technical. 2 The Ghost scene in the play is almost at the precipice of being ‘comic’. â€Å"Ghost music† eerie, unearthly sounds, accompanied by natural wails of humans, animals, of wind and storm ? sometimes sounding so suddenly it jolts the watchers with alarm, often heralds the Ghost’s appearance. â€Å"Stertorous breathing† attended Skinner’s Ghost. A muffled drum accompanied Wilson Barrett’s. Barrault’s silhouetted, slow-motion ghost, Goldsby observed, â€Å"was aided by a muffled drum beat which filled the theatre with the pulsation of the human heart† and a â€Å"high frequency sustained pitch as can be heard on short-wave radio. † Olivier, in his film, also used the heartbeat, and his weird ghost-music was â€Å"painstakingly compounded †¦ from superimposed recordings of fifty women shrieking, fifty men groaning, and twelve violinists scraping their bows across the strings on a single screeching note. † Olivier wanted a sound â€Å"like the lid of hell being opened. † Later theatres, playing Hamlet to audiences increasingly sceptical of the supernatural, have experimented with techniques for enhancing both the mystery and the menace of the ghostly figure. Most simply it has been rendered invisible: is never seen in any of its scenes by the theatre audience, but only by watchers who create its fearful image with their words and faces. It is certainly a difficult ploy for an actor to play the part of something non-existent or metaphysical. We cannot possibly know what the first production of 1603 had, whether they employed the capacity of deus ex machina, but the mere appearance of the ghost on the Elizabethan stage was a feast for the audience. In itself the Ghost is a formidable an ominous figure ? the Elizabethans had never seen a theatre one like it. Possibly Barnardo’s pointing finger picked out the Ghost in the Globe, poised in an â€Å"above† as if suspended in air. In a Swedish staging in 1942, a ghost seemed to float over the walls: â€Å"for the first time on a Stockholm stage the technicians have succeeded in making him a ghost. † (Dagens Nyheter) The concept of time, in the play, and its atrocities modifies itself through both the physical and the psychological dimensions. The stage is set for something sinister and is pushed in parallel to the furtive wordplay. Two tough veteran soldiers, and a clever, sceptical scholar, in the bitter cold, blankly appalled and mesmerized, indeed physically distill’d to jelly by a visitation from the dead. But the Ghost is a manifestation of the suggested supernatural in the play. The whole dome of the play seems open to such visits from the super-terrestrial worlds. If the mere word ‘natural’ is taken in the sense of normality or the general code of behaviour, it defies that definition. â€Å"The Ghost will†, observes Marvin Rosenberg in THE MASKS OF HAMLET , â€Å"have a name: illusion. As long as it is mute, undefined, gesturing ambiguously, it remains darkly and dangerously unfathomable. An image of death amidst life. †3 But time and time again, the political, and mythical nature of the Ghost and Hamlet’s supposed madness has been calculated in terms of degree and balance of the opposites. But the sense of political imagery must not override the personal. Marcellus had told him of the king-like ghost. The Ghost carries a desperate personal need, too, Hamlet being the outlet. It appears as an indication not only of national but cosmic unrest, where the doors of the Earth and Hell are brought face to face yet stumped asunder by intellectual pursuits. The once-sceptical Horatio, haunted by this ghastly entropy of space-time, with unimpeded sophistication, resorts to Plutarchian myth familiar to Elizabethans. The once great Rome, was struck by bizarre omens, a little ere the mightiest Julius fell? The graves stood tenantless, and the sheeted dead Did squeak an gibber in the Roman streets; As stars with trains of fire and dews of blood, Disasters in the sun; and the moist star ? (1. 1. 118-121) Horatio makes to the moon ? Upon whose influence Neptune’s empire stands, Was sick almost to doomsday with eclipse (1. 1. 122-123). The word sick defines this sinister movement further into contemplation: a universe ruled by a Rex Tyrannous ? and this is what happens in Denmark ? And even the like precurse of fear’d events, As harbingers preceding still the fates And prologue to the omen coming on, Have heaven and earth together demonstrated Unto our climatures and countrymen (1. 1. 124 -128). At once, the play is in suspended animation ? two parallel forces of comprehension: the Ghost on one side and Horatio with Marcellus and Barnardo on the other ? almost rub their back in the pursuit of communication. Horatio asks it several questions. But just at the moment of revelation, with a fine melodramatic twist, the cock crows and the Ghost disappear. This withdrawal is not strange, but reiterates the story of the chosen one. The Sphinx’ riddle can only be answered by Oedipus, Excalibur can only be retrieved by king Arthur (King Arthur and the Knights of The Round Table), and Horatio puts it in motion through his speech that makes us look forward into Scene 2: Break we our watch up, and by my advice Let us impart what we have seen tonight Unto young Hamlet; for upon my life This spirit dumb to us, will speak to him (1. 1. 173-176). But before we look further, we must consider the nature of this Ghost. It is, certainly, not a Holy Ghost. It is much more personal than mythological. Ghosts, in the Elizabethan times, were no more motiveless; they had an important social role to play. It appears with a purpose. To the Elizabethans they were instruments of revenge or prophecy. The supernatural was only invoked at the point where natural remedies proved inadequate. This inadequacy is the soil of Hamlet. Throughout the play, in dialogues or in soliloquies, Hamlet harps upon the same strings of inadequacy. The Ghost’s armour is symbolic of the fact that a mere appearance is ‘inadequate’. It asks for a place in Hamlet’s memory: Adieu, adieu, adieu. Remember me. (1. 5. 91). Is it not persuasive enough? There is an instant doubt, whether its darker purpose affects the inner self of Hamlet, which would spring to seek justice with heroic chivalry. Eventually, it doesn’t. Shakespeare is conscious that it is no more the age of the knights. The Wittenberg scholar in Hamlet reasons action. Chivalry, in this Elizabethan world, is replaced by diplomacy. Our Ghost appears, not with the rage of Caesar’s spirit, but with a pitiful face reverberating the loss of value from human life. Although medieval and sixteenth-century treatises on the supernatural indicate a belief in the ability of both angels and demons to walk the earth and to commune with mortals, angelic visits are barely mentioned and all is the matter of demons. Revenge me, it cries. It argues that Hamlet would prove dull if he would not stir in revenging him. Denmark is abused because it has been lied to about â€Å"my death† (my italics). Whatever Hamlet’s beliefs and behaviour in respect to the Ghost as he concludes his first meeting with it, all must be squared with the Prince’s convictions after the mousetrap scene: â€Å"O good Horatio, I’ll take the ghost’s word for a thousand pound† (3. 2. 280-81). The ghost’s truthfulness does not mean necessarily that the spirit is a messenger from heaven. Shakespeare shows explicit intentions on this point, elsewhere. Iago who is a villain himself, does not deck rhetoric’s in his comment on his own demonic nature: When devils will the blackest sins put on, They do suggest at first with heavenly shows. (Othello, 2. 3. 345-46) But if Iago cannot be granted as speaking from the demon’s lair, Banquo at least shows what humans may expect from that world: oftentimes, to win us to our harm, The instruments of Darkness tell us truths, Win us with honest trifles, to betray’s In deepest consequence. (Macbeth, 1. 3. 123-26) For all this knowledge of the lower world Shakespeare found ready corroboration in his sources: The diuel sometimes vttereth the truth, that his words may haue the more credit, and that he may the more easily beguile them. He that would vtter euil wares, doth not only set them foorth in words, but doth also so trim and decke them, that they seeme excellent good. 4 So far so good. But the most significant oddness of the ghost is the direction of his disappearance. We expect an angelic substance to exit upwards. Yet it cries from under the stage. To see it in sinister illumination may also explain the commented strangeness of Hamlet’s retorts to his departed father. The terms of Hamlet’s addresses of his father are perhaps cruel, irreverent at best ? boy, truepenny, fellow, Hic et ubique? , old mole, pioner? yet when he has finished extracting the oath from the others, compassion for his father, dead and perhaps damned, overwhelms him: â€Å"Rest, rest, perturbed spirit! † (1. 4. 183). Unnatural occurrences that accompany the fall or death of kings in Shakespeare’s plays are commonly credited to the great theme of correspondences between the microcosm and the macrocosm which the history of ideas traces from pagan culture to the present. 5 The ghost who prompts Horatio’s observations ushers in a topsy-turvy world. Through usurpation a false king reigns; one from down-under has forced above the legitimate superior, creating an upside-down chain of relations. Through the agency of the ghost ? be it good or evil ? Hamlet is apprised of the serpent â€Å"that did sting [his] father’s life† and â€Å"wears his crown† (1. 4. 40-41). Although the false king, too, destroys Hamlet, his death results primarily from the Prince’s submission to the end that God, not Claudius, has charted for him. He recognizes this premonition: The time is out of joint. O cursed spite, That ever I was born to se it right. (1. 5. 196-197) His preparation for the doomsday is reflected in his readiness: but until he is ready, Hamlet, in order to survive in the topsy-turvy world, must make adjustments. 6 Among them is Shakespeare’s most trusted device: disguise. His disguise is negativity of self-expressions, which is rather psychological than physical. This is negating the order of the mind, through his â€Å"antic disposition†7 a mask: a rhetoric. â€Å"To be† is to be in being; â€Å"not to be† is also to be in being. With this stroke of mastery, Shakespeare has placed the ghost in the correct unity and with proper limit to his function. Hamlet is not driven through the alleys of revenge, blindfolded, which would have marred the potentiality of his character. The ghost is there as an explanatory stimulant to his already reproachful mind. If Claudius is a false king, then there should be a true king. As Dover Wilson demonstrated convincingly, the operative political science in Hamlet is sixteenth-century English monarchical succession. 8 Hamlet is the son of the former king. By law of primogeniture9, the prince is heir to the father’s throne. All begins with Hamlet’s felt but uncertain thought that his uncle has done him wrong: A little more kin, and less than kind. †¦ I am too much in the sun. (1. 2. 65-67) The sheer density of supernaturalism that resorts in Shakespeare’s quill, is apodictic. But as Hamlet proves, supernatural sides with the dramatic modus operandi to utmost precision. Shakespeare may reflect, in Hamlet’s philosophical mood, something beyond the resistance of the scholar to action, to assassinate, to involve in the words, under the well-portrayed supernatural enterprise. It is a psychic inhibition, fallibilism, despair and suffering that he cannot comprehend. He is torn asunder between his knowledge of perceptual judgement and that of a ghost and its narrative. But all knowledge derives by hypothetical reasoning from knowledge of external facts and previous knowledge. Such is with Hamlet. His mind broods over such knowledge as to philosophise action. This incessant struggle between reason and action is reflective of Kant’s theory of practical reason. 10 Within the pathologically affected will of the rational Hamlet we find a conflict of maxims with the practical laws cognised by himself. His rational reasoning is overshadowed by the ambition of a duty, which create its own laws and the process of adjustment is hindered thus. The influence of the supernatural on Hamlet’s maxims and hitherto indecisive mind create imperatives of action, unsupported by his emotions, sobered by rational learning. The depth of Supernatural concepts is abysmal. The elements of psychic and metaphysics, in this play, are too broad to fit into an essay. But having determined the dramatic function and the nature of the treatment in Hamlet, it is time to question it, test its resistances, grasp its openings and its hints, which are never too explicit. The rest is conjecture.

Predicate and Following Sentences free essay sample

Ill tell you the truth. 8. Mrs. Barnes gave Chris a reward for finding her briefcase. 9. Please show me the best way to upholster a chair. 10. Norman handed the bellboy a tip. 11. The University Club awarded Joy a scholarship to study marine biology. 12. I wish you great success. 13. Mr. Brent showed Sam his garden. 14. Mrs. Todd promised me a reward for finding her dog. 15. Dr. Jordan gave his church a large contribution. 16. Ill read the twins a story before their bedtime. 17. Grandfather told me a story about his boyhood. 18. I shall give the Salvation Army my used clothing. 19. The company president gave Mom a watch at her retirement party. 20. Mr. Osborn showed his class some photographs taken on his trip . A. Circle only the direct objects in the following sentences. 1. Jerry was so happy because of his promotion. He hugged everyone in the room. 2. I passed the test. I studied the text for three hours three nights in a row. We will write a custom essay sample on Predicate and Following Sentences or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page I also reviewed my notes. 3. Janet loves all sorts of music. She attends concerts whenever she can. 4. In my house, I feed the dog. However, my wife feeds the cat and waters the plants. 5. His favorite singers are Frank Sinatra and Koko Taylor. I like Mick Jagger and Bette Middler. B. Circle only the indirect objects in the following sentences. 1. He gave me excellent directions to the stadium. 2. We waited for him for two hours, but he never showed up. 3. Yes, I told my class that stupid joke. Most of the students thought it was funny, but a few hated it. 4. The teacher brought the children and her colleagues little gifts for their birthday. 5. I wish he and Marty would be more patient. When they argue, they give me a headache. C. Circle only the subject complements in the following sentences. . He understands how to listen and answer questions. Therefore, he is an excellent teacher. 2. I have been a teacher, a minor league baseball player, and a magazine editor. 3. Those students have terrific study habits. It is a pleasure to have them in class. 4. This test seems really difficult. I feel very unsure of myself. I wish I had studied more. 5. I dress conservatively, but I am a very liberal thinker. Actually, I’m qui te radical. D. Label the direct objects (DO), indirect objects (IO), and subject complements (SC) in the following sentences. Hint: You should have only twelve words marked. ) 1. I don’t understand the concepts in this class. I am totally frustrated. 2. The interviewer mispronounced the actor’s name. He corrected the error and apologized. That was a nice gesture. The actor was not upset. In fact, he handed the interviewer an autographed photo. 3. She felt the patient’s pulse. It felt very weak and erratic. She called the doctor to give a report. Practice: Label the direct objects (DO), indirect objects (IO), and subject complements (SC) in the following sentences. Be careful: some of these sentences contain none of the above. 1. My sister Mary teaches math and physical education at the high school. 2. I gave my younger sister a lovely necklace for her graduation. 3. The teacher was sick yesterday. In fact, she went to the hospital for some tests. 4. I enjoy television. My favorite programs are NYPD and Nature. 5. The students felt great about their essays. The teacher returned them this morning. 6. No, I have not told Karen about the party. I will tell her tonight. 7. My father is quiet and shy. He is an excellent auto mechanic. . Jerry told her the story about the lost dog. It was really a sad story. 9. Usually for breakfast my wife has tea, cereal, and a banana. 10. Marilyn walked to the store, then to the school, and finally to the bank. Identifying Subject Complements. Underline the subject complement in each of the following sentences. Look for the linking verb to help you find the subject complement. Example: Cheddar cheese is orange. Jo hn feels sick today. 1. The mongoose, introduced to Puerto Rico to kill poisonous snakes, has become the island’s most annoying predator. 2. Why are many of our folk heroes outlaws? 3. The chlorine in the pool smelled harsh. 4. This bread tastes moldy to me. 5. The hungry beggar felt too faint to hold out his cup. 6. The caged tiger gets especially restless during storms. 7. After my yoga exercises, I feel almost weightless. 8. Mahatma Gandhi always remained peaceful, despite his opponents’ most outrageous provocation. 9. One of a mail carrier’s occupational hazards is the overzealous watchdog. 10. Our captain’s last words to us sounded prophetic. 11. The sunflowers by the gate grow taller every day. 2. The class gift will be a new set of curtains for the auditorium stage. 13. The achievements and courage of Dr. Martin Luther King have become part of our American heritage. 14. A horse’s staggering gait can be a sign of swamp fever. 15. Her story rings true. 16. According to Dr. W. H. Sheldon’s classification of humans into physical types, the thin, slender- boned ectomorph is introspecti ve. 17. Are you nervous about making the speech? 18. The dirigible is lighter than air. 19. Is his pulse normal? 20. The moon looked close enough to touch.

Amur Leopard Facts

Amur Leopard Facts The Far Eastern or Amur leopard (Panthera pardus orientalis) is one of the worlds most endangered cats. It is a solitary, nocturnal leopard with a wild population estimated at over 84 individuals who mostly reside in the Amur River basin of eastern Russia with a few scattered in neighboring China and in a relatively new refuge established in 2012. They are particularly vulnerable to extinction because Amur leopards have the lowest levels of genetic variation of any leopard subspecies. Fast Facts: Amur Leopard Scientific Name: Panthera pardus orientalisCommon Names: Amurland leopard, Far Eastern leopard,  Manchurian leopard, Korean leopardBasic Animal Group:  MammalSize: 25–31 inches at the shoulder, 42–54 inches longWeight: 70–110 poundsLifespan: 10–15  yearsDiet:  CarnivoreHabitat:  Primorye region of southeastern Russia and northern ChinaPopulation:  More than 80Conservation  Status: Critically Endangered Description The Amur leopard is a subspecies of leopard with a thick coat of long, dense hair varying in color from creamy yellow to rusty orange, depending on their habitat. Amur leopards in the snowier Amur River Basin of Russia develop lighter coats in the winter and tend to have more cream-colored coats than their Chinese kin. Their rosettes (spots) are more widely spaced with thicker black borders than other subspecies of leopards. They also have larger legs and wider paws than other subspecies, an adaptation that facilitates movement through deep snow.   Both males and females range in height between 25 to 31 inches at the shoulder and are typically 42 to 54 inches long. Their tales measure approximately 32 inches in length. Males are typically heavier at 70 to 110 pounds while females typically weigh 55 to 75 pounds.   Thomas Kitchin Victoria Hurst​/Getty Images Habitat and Range Amur leopards can survive in temperate forest and mountain regions, keeping mostly to south-facing rocky slopes in winter (where less snow accumulates). Individuals territories can range from 19 to 120 square miles, depending upon age, sex, and prey density- the latter of which has greatly diminished in recent years, though they are on increase in protected areas. Historically, Amur leopards have been found in eastern China, southeastern Russia, and throughout the Korean Peninsula. The first known documentation was a skin found by German zoologist Hermann Schlegel in 1857 in Korea.  More recently, the few remaining leopards are scattered throughout approximately 1,200 square miles in the area where the borders of Russia, China, and North Korea meet the Sea of Japan. Today, Amur leopards are increasing in numbers, due to the creation of protected areas and other conservation efforts. Diet and Behavior The Amur leopard is a strictly carnivorous predator that primarily hunts roe and sika deer but will also eat wild boar, Manchurian wapiti, musk deer, and moose. It will opportunistically prey on hares, badgers, raccoon dogs, fowl, mice, and even young Eurasian black bears. Reproduction and Offspring Amur leopards reach reproductive maturity between the ages of two and three years. Females estrus period last from 12 to 18 days with gestation taking approximately 90 to 95 days. Cubs are typically born from the end of March through May and weigh a little over one pound at birth. Like domestic cats, their eyes remain closed for about a week and they begin to crawl 12 to 15 days after birth. Young Amur leopards have been reported to remain with their mother for up to two years. Amur leopards have been known to live for up to 21 years in captivity, though their lifespan in the wild is typically 10 to 15 years. Kuzmichstudio/Getty Images Conservation Status According to the World Wildlife Fund, Amur leopards received a safe haven in 2012 when the government of Russia declared a new protected area. Called Land of the Leopard National Park, this marked a major effort to save the world’s rarest cat. Extending nearly 650,000 acres it includes all of the Amur leopard’s breeding areas and about 60 percent of the critically endangered cat’s remaining habitat. In addition, conservationists have been successful in reducing illegal and unsustainable logging practices and facilitating trade between companies committed to responsible forestry practices. In 2007, WWF and other conservationists successfully lobbied the Russian government to reroute a planned oil pipeline that would have endangered the leopards habitat. The IUCN Species Survival Commission has considered Amur leopards  Critically Endangered (IUCN 1996)  since 1996. As of 2019, more than 84 individuals remain in the wild (mostly in protected areas) and 170 to 180 live in captivity. The primary causes for their low population are habitat destruction from commercial logging and farming from 1970 to 1983 and illegal poaching for fur over the last 40 years. Fortunately, conservation efforts by organizations like the World Wildlife Fund and the Amur Leopard and Tiger Alliance (ALTA) are working to recover the species from extinction. Threats Although human interference plays a key role in Amur leopards endangered status, their low level of genetic variation due to recent dwindling population size has led to many health complications including reduced fertility.   Habitat destruction:  Between 1970 and 1983, 80 percent of the Amur leopards habitat was lost due to logging, forest fires, and agricultural land conversion projects (this loss of habitat also affected the leopards prey species, which have become increasingly scarce as well).Human Conflict:  With less wild prey to hunt, leopards have gravitated to deer farms where they have been killed by farmers.Poaching:  The Amur leopard is illegally hunted for its fur, which is sold on the black market. Habitat loss has made it easier to locate and kill leopards within the past 40 years.Small Population Size:  The Amur leopards critically low population is at risk from disease or environmental catastrophes that could wipe out all remaining individuals.Lack of Genetic Variation:  Because there are so few individual leopards left in the wild, they are subject to inbreeding. Inbred offspring are prone to health problems, including reduced fertility which further reduces the populations cha nce of survival. Though these issues are being addressed and the number of Amur leopards has increased, the species is still considered to be critically endangered. Amur Leopards and Humans The Amur Leopard and Tiger Alliance (ALTA) works in close cooperation with local, regional, and federal organizations to protect the regions biological wealth through conservation, sustainable development, and local community involvement. They maintain four anti-poaching teams with a total of 15 members in the Amur leopard range, monitor the Amur leopard population through snow track counts and camera trap counts, restore leopard habitats, support ungulate recovery, and run a media campaign to create awareness about the Amur leopards plight. The World Wildlife Fund (WWF) has established anti-poaching teams and environmental education programs to increase appreciation for the leopard among local communities within the leopards range. WWF also implements programs to stop the traffic in Amur leopard parts and to increase the population of prey species in the leopards habitat such as the 2003 Forest Conservation Programme in the Russian Far East Ecoregion Complex, the 2007 lobbying effort to reroute a planned oil pipeline, and the 2012 establishment of a large refuge for Amur leopards, tigers, and other endangered species. Sources â€Å"About the Amur Leopard.†Ã‚  About the Amur Leopard | Russian Geographical Society, www.rgo.ru/en/projects/protection-endangered-species-amur-leopard/about-amur-leopard.â€Å"Amur Leopard.†Ã‚  WWF, World Wildlife Fund, www.worldwildlife.org/species/amur-leopard#.â€Å"Amur Leopard-Worlds Rarest Cat-Doubles in Population.†Ã‚  WWF, World Wildlife Fund, 23 Feb. 2015, www.worldwildlife.org/stories/amur-leopard-world-s-rarest-cat-doubles-in-population.

2009 Chrysler Fiat Strategic Alliance

The company had no choice but to look for a partner. During this process, Chrysler explored the possibility of a tie-up with GM, Ford, Volkswagen, Tata Motors, Nissan and Fiat. Eventually Chrysler decided on creating a strategic alliance where Fiat agreed on taking a 20 percent stake in Chrysler. In the next five years, the tie-up may increase Fiat’s ownership of Chrysler to 35 percent. Both companies show compatibility in their product portfolios, global operations, and technology sharing areas. Case ObjectivesThe primary objective of this case is to analyze and discuss Chrysler’s 2009 strategic alliance with Fiat and its current and future prospects. Issues that are at the helm of this tie-up are technology sharing, global integration, quality control, and reorganization of brand portfolios. Students need to look at the intricacies of strategic alliances between two or more companies as well. As of May 2009, Chrysler is going through its Chapter 11 and corporate restructuring in the U. S. The company continues to shrink in terms of its manufacturing and global operations.Suggested Teaching Approach and Student Assignments Since the case is timely, it is recommended that class discussions should be based on the companies’ histories and their evolutionary growth (see Tables 2 and 3). Also important are topics such as strategic alliances, auto industry’s technology platforms, and brand portfolios. The questions included in the case for class discussion will require Web-based and library research on the part of students. It is recommended that the instructors provide a supplementary list of references on the auto industry (see: WardsAuto. om, JDPower. com, Automotive News, Google Search, Business Source Complete, Hoovers. com, Reuters. com, Value Line, Standard Poor’s Industry Surveys, etc. ). This will help students to be fully prepared for the case. Finally, students should be encouraged to visit Chrysler, Fiat, and other auto manufacturers’ Web sites before discussing this case in the classroom. Discussion Questions Answers Question 1. What are your views of the 2009 Chrysler-Fiat strategic alliance and its future prospects in the auto industry? Question 2. Analyze and evaluate Chrysler and Fiat’s strengths and weaknesses before and after their 2009 strategic alliance. Question 3. Compare and contrast Chrysler and Fiat with five other global auto manufacturers (GM, Ford, Toyota, Volkswagen and Daimler) in the areas of global operations and manufacturing issues. Question 4. Analyze Chrysler and Fiat’s brand portfolios in the world auto industry. How do you see both companies revamping and overhauling their brands in the short- (1-2 years) and long-terms (5-6 years). Question 5. What did you learn from the Chrysler-Fiat Strategic Alliance regarding managing multinationals in the changing global business? Case Analysis The Chrysler-Fiat strategic alliance will provide a meaningful opportunity to both companies regarding dealing with survival and expanding their markets in Europe and North America. As of 2009, it is evident that the companies are off to a good start. Both firms have marketable brands in their market segments but lack a collective effort. The case is a good example in the area of cross-border alliances that take place between two or more companies.Since 2008, Fiat has actively sought a partner in North America because of the attraction of the market. This is also attributed to the auto industry’s consolidation, restructuring, and cost-cutting activities. Chrysler and Fiat ended up seeking an alliance because of their compatibility, product portfolios, and markets. In Chrysler’s case, the main motive was to seek a partner who could help strengthen its financial problem regarding new technologies, markets, and quality standards.